Fig 2.

Substrate profiles of the wild-type LAE2, LAE5, and LAE7 α/β hydrolases from Lake Arreo against a set of structurally diverse ester substrates. LAE2, LAE5, and LAE7 were the α/β hydrolases characterized by a restricted substrate spectrum. Reactions (30°C and pH 7.2) were performed as described for the α-naphthyl and triacylglycerol esters (Table 1) and were monitored during 15 min, and the absorbance of p-nitrophenol at 404 nm was recorded and plotted. For best and/or representative substrates, specific activities (U/mg) were calculated as described in Table 1 and Materials and Methods and are shown on the top of bars. In all cases, three independent experiments were performed for each parameter, and graphs were plotted using mean values; the standard deviations were less than 5%.