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Substrate specificity of the wide-substrate-spectrum LAE1, LAE3, LAE4, and LAE6 α/β hydrolases from Lake Arreo against a set of structurally diverse ester substrates. LAE1, LAE3, LAE4, and LAE6 were the hydrolases characterized by the widest substrate spectrum. Reaction conditions (30°C and pH 7.2) and activity parameter determinations were as described in Table 1 and the legend to Fig. 2. M2Cl3HP, methyl-2-chloro-3-hydroxypropionate.
