Fig 1.

PFGE of nondigested genomic DNA from C. botulinum type E (A) and Southern hybridization with probes specific for 16S rrn (B), botE (C), and orfX1 (D). Strain numbers correspond to those listed in Table 1. Each strain was loaded with genomic DNA (left) and S1 nuclease-treated genomic DNA (right) in two adjacent gel wells. For the strains shown in lanes 14, 23, 27, and 32, the marker (M) was loaded in the lane between the genomic DNA and S1 nuclease-treated genomic DNA. The white arrows indicate plasmid bands (A), and the black arrows indicate plasmid bands hybridized with probes specific for botE (C) and orfX1 (D).