Fig 3.

Comparison of product data from the feed phase of cultivations performed with the plasmid-based system [BL21(DE3)(pET30aSOD), open circles] and the plasmid-free system [BL21(DE3)::TN7<T7-SOD>, filled circles]. Determination of the induction of recombinant gene expression was conducted at feed hour 7. (A) Specific (spec) concentrations of SOD (soluble and insoluble) in mg per gram CDM. (B) Specific (spec.) product formation rates in mg per gram CDM and hour. The dashed line indicates the time point of induction. Data shown are derived from three independent replicate experiments (n = 3).