Table 1.

Phenotypic and genetic evidence for aromatic-compound catabolism in S. stellata E-37

Carbon substrate	Concn(s) (mM)	Growthb	PcaHG sp actc	Catabolic gene(s) in E-37	Locus tag(s)f
Acetate	3.5, 7, and 25	+	0.017 ± 0.008	TCA cycle (gltA, acnA, icd, sucABCD, shdABCD, fumC, and mdh)	SSE37_06879, SSE37_25138, SSE37_06274, SSE37_11324, SSE37_11329, SSE37_11294, SSE37_11309, SSE37_11244, SSE37_11229, SSE37_11254, SSE37_11249, SSE37_05510, SSE37_11289
Benzoate	0.5, 1, and 2	+	0.030 ± 0.016	bcl-boxDCBAE	SSE37_24404, SSE37_24409, SSE37_24419, SSE37_24424, SSE37_24439, SSE37_24444
Caffeate	1, 2, and 3	−	ND	hcaABC	SSE37_01000, SSE37_01050, SSE37_12324
Catechol	1, 2, and 3	−	ND	xylE	SSE37_12149
Chlorogenate	1, 2, and 3	−	ND	hcaG, hcaABC, and pobA	SSE37_18822, SSE37_01000, SSE37_01050, SSE37_12324, SSE37_18837
Ferulate	2	+	(+)d	Unke and vanAB	SSE37_02815, SSE37_00265
Gentisate	1, 2, and 3	−	ND	nagILK	SSE37_02745g, SSE37_02750, SSE37_02755
Homogentisate	NDa	ND	ND	hmgAB and hmgC	SSE37_24544, SSE37_24549, SSE37_14734
Homoprotocatechuate	ND	ND	ND	hpaBC and hpaDEF	SSE37_00435, SSE37_00425, SSE37_23379, SSE37_23374, SSE37_23369
p-Coumarate	2	+	1.205 ± 0.702	hcaC and pobA	SSE37_12324, SSE37_18837
p-Hydroxybenzoate	0.5, 1, and 2	+	0.477 ± 0.051	pobA	SSE37_18837
Phenylacetate	2	+	0.005 ± 0.017	paaABCDEZ and paaIJK	SSE37_00390, SSE37_00385, SSE37_00380, SSE37_00375, SSE37_00370, SSE37_00360, SSE37_15758, SSE37_15753, SSE37_15748
Protocatechuate	2	+	0.632 ± 0.141	pcaCHG, pcaDB, pcaIJ, and pcaF	SSE37_18842, SSE37_18847, SSE37_18852, SSE37_21470, SSE37_21460, SSE37_23014, SSE37_23019, SSE37_16533
Quinate	2	+	0.694 ± 0.140	qsuBCD	SSE37_03985, SSE37_06077, SSE37_03980
Salicylate	1, 2, and 3	−	ND	salA	SSE37_01015
Vanillate	2	+	(+)	vanAB	SSE37_02815, SSE37_00265

^aND, not determined.

^bGrowth was assessed by monitoring the optical density at 540 nm of cultures in MBM.

^cPcaHG activity was scored as “−” or “+” if the specific activity was <0.04 or >0.50 μmol pca min⁻¹ μg protein⁻¹, respectively.

^d“(+)” indicates that specific activity was not quantified but levels were >0.50 μmol pca min⁻¹ μg protein⁻¹.

^eUnk, gene(s) involved in the pathway is unknown.

^fLocus tags unique to the E-37 genome are listed in respective order of the catabolic genes.

^gA disparately located nagI duplicate exists (SSE37_02425).