Time course of the substrate consumption and the product formation by transfucosylation catalyzed by α-l-fucosidases AlfB (A) and AlfC (B). ●, p-nitrophenyl-α-l-fucopyranoside; ◆, p-nitrophenol; ▼, l-fucose; ■, fucosyl-α-1,3-N-acetylglucosamine; □, fucosyl-α-1,6-N-acetylglucosamine. Data from a representative experiment are shown.