Fig 3.

Effect of salinity on the expression of different terminal oxidase genes. H. halophilus was grown in NB-Mg²⁺medium in the presence of 1.0, 2.0, and 3.0 M NaCl to the exponential growth phase. RNA was isolated and transcribed into cDNA as described previously (19). The cDNA was then used for quantitative real-time PCR analyses. Real-time PCR analysis was done with three independent physiological parallels to ensure statistical relevance. The x-fold copy numbers were determined using the value of the cbaB sample as the reference. Error bars show standard deviations.