Table 1.
pHe | Mean value ± SD for: |
||||
---|---|---|---|---|---|
pHi | ΔpH | Δψ (mV) | Δμ̃H+ (mV) | ATP (nmol/mg) | |
6.6 | 6.52 ± 0.07 | −0.08 ± 0.07 | −194 ± 9 | −190 ± 13 | 1.5 ± 0.1 |
7.4 | 7.24 ± 0.06 | −0.19 ± 0.06 | −201 ± 9 | −190 ± 13 | 3.8 ± 0.2 |
8.0 | 7.41 ± 0.12 | −0.59 ± 0.12 | −218 ± 4 | −183 ± 11 | 3.2 ± 0.3 |
8.5 | 7.86 ± 0.08 | −0.64 ± 0.08 | −220 ± 5 | −182 ± 10 | 2.8 ± 0.2 |
8.9 | 7.98 ± 0.02 | −0.96 ± 0.02 | −188 ± 12 | −133 ± 13 | 1.3 ± 0.3 |
Cell suspensions were incubated in 0.05 M Tris-HCl, pH 7.5, containing 0.05 M MgSO4 and 1 M NaCl. The bioenergetics parameters were determined as described in Materials and Methods. pHe, external pH; pHi, internal pH.