Table 2.

Amount of energy spilling following changes in assumed parameter values for spilling calculations

Current assumption	Alternate assumption	Note (reference for alternate assumption)	Energy spilling (% of heat production) for dosage with:		Differences
			5 mM glucose	20 mM glucose
All	None (no changes)		6.3	21.1	14.8
Maintenance functions estimated by endogenous metabolisma	Functions estimated by energy use of rumen bacteria extrapolated to growth rate = 0b	See text	14.7	25.4	10.6
Endogenous metabolism declines by 7.3%/h	Metabolism is constant	Hypothetical	4.5	18.3	13.8
0 mol ATP/mol glucose transported	0.33 mol ATP/molc	Cost of ion–driven transport (20)	2.6	16.4	13.7
4 mol ATP/2 mol propionated	2 mol ATP/2 mole	Yield of acrylate pathway (20)	2.5	16.2	13.7
1 mol ATP/mol CH4f	0.3 mol ATP/molg	Lowest yield in reference 48	3.5	17.9	14.4
	1.5 mol ATP/molh	Highest yield in reference 48	8.0	23.1	15.1
Reserve polysaccharide has chain length of 15 residues and is polymer of glucosei	Polymer of fructosej	Hypothetical	7.8	22.8	15.1
	Polymer of galactosek	Hypothetical	9.0	24.3	15.3
	Polymer of mannosel	Hypothetical	8.0	23.1	15.1
	8 residuesm	Lowest measurement (26)	6.1	20.8	14.7
	25 residuesn	Highest measurement (24)	6.4	21.2	14.8
Thermodynamic properties of gas reactants assume reactants in gaseous stateo	Aqueous statep	Vaporization of aqueous state can be slow (49)	2.4	16.3	13.9
Thermodynamic properties of species assume pH 6.80q	pH 6.65r	Lowest pH measured in current study	6.3	21.1	14.8

^a33.7 mW/g cellular protein (SEM = 2.7 mW/g; n = 8; see text).

^b28.0 mW/g cellular protein (see text) (adapted from reference 33).

^cΔ_rATP for glucose-utilizing reactions decrease by 0.33; see Table 1 for current values and equation 4 for application.

^dΔ_rATP(GlcPr) = 4; see Table 1 for current value and equation 4 for application.

^eΔ_rATP(GlcPr) = 2; see equation 4 for application.

^fΔ_rATP(CO₂CH₄) = 1; see Table 1 for current value and equation 4 for application.

^gΔ_rATP(CO₂CH₄) = 0.3; see equation 4 for application.

^hΔ_rATP(CO₂CH₄) = 1.5; see equation 4 for application.

ⁱΔ_fH′⁰_RC = −973.94; see Table S3 in the supplemental material for the current value and equation 1 for application.

^jΔ_fH′⁰_RC = −970.21; see equation 1 for application.

^kΔ_fH′⁰_RC = −967.01; see equation 1 for application.

^lΔ_fH′⁰_RC = −969.71; see equation 1 for application.

^mΔ_fH′⁰_RC = −974.47; see equation 1 for application.

ⁿΔ_fH′⁰_RC = −973.68; see equation 1 for application.

^oSee current values in Table S3 in the supplemental material; see equations 1, 2, and 3 for application.

^pSee alternate values in Table S3 in the supplemental material; see equations 1, 2, and 3 for application.

^qSee the current values of properties in Table S2 in the supplemental material; see text for application.

^rFor brevity, alternate values are not shown.

^sValue for 20 mM glucose − value for 5 mM glucose.