Fig 2.
Contents (A) and 3HHx monomer fractions (B) of PHA accumulated by E. coli LS5218 transformants harboring wild-type and individual A479X mutant PhaCCs. E. coli LS5218 transformants were cultivated in 100 ml of M9 medium containing 0.25% (wt/vol) dodecanoic acid and 0.4% (vol/vol) Brij 35 for 72 h at 30°C. Ampicillin and kanamycin were supplemented at final concentrations of 100 μg/ml and 50 μg/ml, respectively, for plasmid maintenance. The data shown are means of triplicate experiments. Mean values indicated by different letters are significantly different (Tukey's HSD test; P < 0.05). The error bars indicate standard deviations. (C) Comparison of the expression levels of wild-type and A479X mutants of PhaCCs in E. coli LS5218 transformants by Western blotting. A total of 40 μg of protein was used for the analysis.