Fig 3.
Correlation between in vitro and in vivo activities of wild-type and A479X mutants of PhaCCs. Each letter of the alphabet represents 1 of the 19 amino acid residues replacing the original one (WT) at position 479 in phaCCs. The data points show a linear correlation (dotted line). In vitro activity was determined by an enzymatic assay of crude synthase extracts (40 μg) using (R)-3HB-CoA as the substrate (600 μM). One unit of enzyme activity is defined as the amount of enzyme that catalyzed the release of 1 μmol CoA/min. In vivo activity was determined from freeze-dried cells via gas chromatography. The data shown are means of triplicate experiments. The error bars indicate standard deviations.