Table 1.

In vitro and in vivo activities of wild-type and A479X mutants of PhaC_Cs^a

PHA synthaseb	PHA content (wt%)c	Relative PHA content (%)d	PHA synthase activity (U/g)e	Relative PHA synthase activity (%)f
Wild type	35.6 ± 2.9	100 ± 8	121 ± 9	100 ± 7
A479C	19.0 ± 6.9	53 ± 19	83 ± 2	69 ± 2
A479E	3.5 ± 1.8	10 ± 5	31 ± 6	26 ± 5
A479G	58.2 ± 2.8	163 ± 8	180 ± 20	149 ± 17
A479N	0.9 ± 0.1	2 ± 0.2	24 ± 4	20 ± 3

^aThe data shown are means of triplicate experiments.

^bCrude extract from E. coli LS5218 transformants harboring wild-type and individual A479 mutant PhaC_Cs grown on LB medium containing 100 μg/ml of ampicillin and 50 μg/ml of kanamycin for 9 h at 30°C.

^cDetermined from freeze-dried cells via gas chromatography.

^dPercentage relative to PHA accumulation by wild-type PhaC_Cs in the E. coli transformant.

^eOne unit of enzyme activity is defined as the amount of enzyme that catalyzed the release of 1 μmol CoA per minute.

^fPercentage relative to the activity of wild-type PhaC_Cs for the polymerization of (R)-3HB-CoA.