Table 2.
Molecular weights of P(3HB) and P(3HB-co-3HHx) synthesized by E. coli LS5218 transformants harboring wild-type and various A479X mutant PhaCCsa
| PHA synthase | 3HHx fraction (mol%) | Mw (103) | Mn (103) | Mw/Mnb |
|---|---|---|---|---|
| Wild type | 1.6 | 168 | 52 | 3.2 |
| A479C | 0.7 | 223 | 73 | 3.1 |
| A479D | 1.2 | 240 | 66 | 3.6 |
| A479E | 0 | 272 | 79 | 3.4 |
| A479F | 0 | NDc | ND | ND |
| A479G | 2.6 | 113 | 40 | 2.8 |
| A479H | 1.9 | 261 | 74 | 3.5 |
| A479I | 0.8 | 190 | 71 | 2.7 |
| A479K | 0 | ND | ND | ND |
| A479L | 0 | 242 | 67 | 3.6 |
| A479 M | 5.4 | 166 | 46 | 3.6 |
| A479N | 0 | ND | ND | ND |
| A479P | 1.0 | 196 | 56 | 3.5 |
| A479Q | 2.5 | 175 | 62 | 2.8 |
| A479R | 0 | ND | ND | ND |
| A479S | 6.6 | 196 | 54 | 3.6 |
| A479T | 4.4 | 187 | 49 | 3.8 |
| A479V | 2.0 | 170 | 62 | 2.7 |
| A479W | 0.8 | 195 | 56 | 3.5 |
| A479Y | 1.1 | 215 | 59 | 3.6 |
a
Cells were cultivated in 100 ml of M9 medium containing 0.25% (wt/vol) dodecanoic acid, 0.4% (vol/vol) Brij 35, 100 μg/ml of ampicillin, and 50 μg/ml of kanamycin for 72 h at 30°C.
b
Polydispersity index.
c
ND, not determined.