Fig 1.

Antisera against B. burgdorferi B31 RevA recognizes Rev proteins across the Lyme borreliae. (A) Coomassie brilliant blue-stained 12.5% acrylamide gel of recombinant Rev (rRev) proteins. The positions of molecular weight markers are indicated to the left of the gel. B31 is the type strain of B. burgdorferi (28). N40 is an B. burgdorferi strain isolated from a tick (47). Strain 297, originally isolated from cerebrospinal fluid from a Lyme disease patient with meningitis (48), has two separate copies of RevA (19). PBi is a European isolate of B. garinii. (B) Western blot of the gel in panel A with affinity-purified antibody to RevA from strain B31. Mass spectrometric analysis (University of Kentucky Center for Structural Biology Protein Core Facility) indicated that the higher-molecular-weight bands present in most lanes are also RevA, suggesting that RevA may form homomultimers. Note that the affinity-purified antibodies to RevA from strain B31 do not cross-react with human plasma fibronectin.