Fig 2.

Purification of conjugates. Hydrophobic interaction chromatography (phenyl-Sepharose 6 Fast Flow High Sub) of PS6B-rPspA1 conjugate: PS6B-rPspA1 produced by reductive amination (top) and PS6B-OCT-mPspA1 produced by conjugation using DMT-MM (bottom). The chromatograms of PS6B and free rPspA1 are displayed in both panels. The amounts of free PS6B and conjugated PS6B loaded in the column were 5 mg and 25 mg, respectively. Elution was with a decreasing gradient from 1 M to 0 M (NH₄)₂SO₄. The absorbance (Abs) at 490 nm and the absorbance at 280 nm correspond to the measurements for PS6B and rPspA1, respectively.