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Targeting properties of a synthetic charged repeat motif. (A) We generated a synthetic charged repeat motif and fused it to a GFP reporter generating a 55-kDa fusion protein (sCRMP::GFP; Western blot in panel B). (C) The protein associates with tubulin-associated structures, such as microtubules and basal bodies, and is further targeted to the cilia. αTub, anti-tubulin. (D) Detail showing the localization of sCRMP to the contractile vacuolar pore. Scale, 10 μm.
