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Constructs of chimeric GFPs. Chimeric GFPs were constructed by fusing the N-terminal signal peptide sequence of AfChiB1 and different GPI-anchor signals derived from A. fumigatus GPI proteins with the N and C termini of GFP, respectively. The ω-site amino acid is shown in boldface. The ω-minus sites are underlined. White and gray bars indicate N-terminal signal sequences from AfChiB and GFP, respectively.
