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Examination of chimeric GFPs in membrane (A), cell wall (B), and culture medium (C) of A. fumigatus. The same amount of proteins in the membrane, cell wall, or culture supernatant of strain MP1-21, GEL1-6, or ECM33-1 was analyzed by SDS-PAGE (left side of each panel) and Western blotting (right side of each panel). Anti-GFP polyclonal antibodies (Clontech) at a 1:1,000 dilution were used for Western blotting.
