Fig 5.

Expression of FlfA in G. anatis. Western blotting demonstrated recognition of a protein band corresponding to FlfA by anti-FlfA immune serum in whole-cell extracts prepared from five of the eight investigated G. anatis strains, including G. anatis 12656-12 (lane 1). The positions of molecular mass standards (in kDa) are shown on the left. Whole-cell extract equivalent to OD₂₆₀ between 4 and 8 in 10 μl was loaded to each lane. Lane 1, G. anatis 12656-12; lane 2, G. anatis 7990; lane 3, G. anatis Avicor; lane 4, G. anatis CCM5995; lane 5, G. anatis IPDH 697-78; lane 6, G. anatis F149; lane 7, G. anatis 10672/6; lane 8, G. anatis 4895.