Table 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant characteristicsa | Source or reference |
|---|---|---|
| Strains | ||
| G. anatis | ||
| 12656-12 | Wild-type strain; biovar 4, isolated in 1981 (Denmark) | 51 |
| ΔflfA mutant | flfA deletion mutant of G. anatis 12656-12; Kmr | This study |
| F149T | Wild-type strain; isolated in 1979 (Denmark) | 2 |
| 10672/6 | Wild-type strain; biovar 1 (Denmark) | 2 |
| 4895 | Wild-type strain; biovar 4 (Mexico) | 52 |
| 7990 | Wild-type strain; biovar 1 (Mexico) | 52 |
| Avicor | Wild-type strain; biovar 3 (Mexico) | 52 |
| CCM5995 | Wild-type strain; biovar 20, isolated in 1978 (Czech Republic) | 2 |
| IPDH 697-78 | Wild-type strain; biovar 15, isolated in 1978 (Germany) | 2 |
| E. coli | ||
| TOP10 | E. coli host strain for routine cloning | Invitrogen |
| DH5α | E. coli host strain for routine cloning | Invitrogen |
| BL21 CodonPlus | E. coli strain for expression of recombinant proteins | Stratagene |
| Plasmids | ||
| pENTR/SD/D-TOPO | Directional cloning vector for entry to the Gateway System | Invitrogen |
| pDEST17 | Gateway Destination vector (Ampr, N-terminal His6 fusion tag) | Invitrogen |
| pDEST17flfA | FlfA expression vector (Ampr, N-terminal His6 fusion tag) | This study |
| pBluescript II KS(+) | General purpose cloning vector (Ampr) | Stratagene |
| pUC4K | Vector carrying the Kmr cassette from Tn903 | 53 |
| pBSΔflfA | pBluescript II KS(+) containing flanking regions of the flfA gene (Ampr) | This study |
| pBSΔflfA-Kmr | Plasmid for flfA deletion with flanking regions of the gene cloned on either side of the Kmr cassette from pUC4K into pBluescript II KS(+) (Ampr Kmr) | This study |
a
Ampr, ampicillin resistance; Kmr, kanamycin resistance; His, histidine.