Table 2.

Primers used in this study

Primer	Sequence (5′–3′)a	Function	Amplicon
1162F	CACCATGGGTGCATTTGCGGATGATCC	PCR amplification of FlfA	flfA
1162R	TATTCGTATGCGATAGTATAGTTC	PCR amplification of FlfA	flfA
FlfA_UF_n2	GGCTCTAGACTCCAAAATGGAGTAGTGAT	Deletion mutant generation	Upstream fragment of FlfA
FlfA_UR_n2	TATCCCGGGATCTGTTGCAGATTGACAAC	Deletion mutant generation	Upstream fragment of FlfA
FlfA_DF	TTACCCGGGAAGCAAGAAAACAGCCACA	Deletion mutant generation	Downstream fragment of FlfA
FlfA_DR	GGACTCGAGGCTCGGTGAAATGGTTAAT	Deletion mutant generation	Downstream fragment of FlfA
FlfA_UF2	ACCTTATGTATGCTCCTATG	Mutant confirmation
FlfA_DR2	AAAAATCGGGCAGGAAATCT	Mutant confirmation

^aThe introduced TOPO cloning site (CACC) and start codon (ATG) is indicated in boldface. Introduced restriction sites are underlined.