Fig 4.

Motility-deficient P. aeruginosa is less efficient at induction of cell death. (A) BMDC from C57BL/6 mice were infected with wild-type PA14 or the motAB motCD mutant at an MOI of 5. Bacteria were gated out, and the percentage of PI⁺ cells was determined. (B and C) Gated PI⁺ cells upon infection with WT or the indicated deletion mutant of P. aeruginosa at an MOI of 50. (B) Representative histograms of data represented in panel C showing gated PI⁺ cells when BMDC were not infected (filled gray) or were infected with wild-type PA14 (solid black line) or motAB motCD bacteria (dashed line) at an MOI of 50. Data are representative of at least two independent experiments (n ≥ 4) and expressed as means ± standard deviations. ***, P ≤ 0.001; **, P ≤ 0.01; *, P ≤ 0.05.