Fig 5.

Loss of flagellar motility leads to reduced release of active IL-1β and caspase-1. BMDC from C57BL/6 mice and, where indicated, caspase-1^−/− BMDC were infected with wild-type or the indicated mutant of PA14 at an MOI of 50. The uncleaved pro- and biologically active cleaved forms of IL-1β (A) and caspase-1 (B) were analyzed in the supernatants by Western analyses. Cell lysates for the corresponding samples were analyzed for β-actin. BMDC treated with LPS and ATP were included as a positive control.