Fig 5.

Effect of MMA-tet on Stx1-induced protein synthesis inhibition. Vero cells cultured in DMEM supplemented with 10% fetal calf serum were pretreated with each amount of MMA-tet for 1 h and then treated with Stx1 (100 pg/ml) for 1.5 h. After extensive washing, the cells were incubated with 2 μCi/well [³H]Leu in Leu-free MEME at 37°C for 30 min. The relative amounts of radioactivity incorporated into cellular proteins are presented as percentages of the control value without Stx1 (mean ± standard error, n = 3; *, P < 0.05, Tukey's test).