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Levels of IL-17 in spleens of IL-10+/+ and IL-10−/− mice infected i.d. with F. tularensis LVS. (Top panel) RNA transcript levels; (bottom panel) protein levels. Mice were infected with 2.5 × 105 CFU of LVS/mouse. Spleens were harvested from three mice/group on days 1, 3, 5, 7, 14, and 21 after infection, homogenized, and analyzed for IL-17 by RT-PCR and ELISA, respectively. **, P < 0.01; ***, P < 0.001; ****, P < 0.0001 (compared to IL-10−/− mice).
