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SDS-PAGE and Western blotting of CPBAs1. (A) SDS-PAGE analysis of recombinant CPBAs1 that was expressed in E. coli Origami after purification with Ni-NTA agarose. (B) Western blotting of midgut-extracted protein cocktail (lane 1) and purified recombinant CPBAs1 (lane 2). To perform Western blotting, rabbit anti-CPBAs1 polyclonal antibody and horseradish peroxidase-conjugated anti-rabbit antibody were used as first and second antibodies, respectively.
