Table 1.
List of the primers that were designed and used in this studya
| Primer name | Sequence |
|---|---|
| Linker | 5′-GAGATTTGAATCTTGCTTCTGGGCCCTCTATTGTCATTGTCTTTTTTTTTTTTTTTTT-3′ |
| Outer | 5′- GAGATTTGAATCTTGCTTCTG-3′ |
| Inner | 5′-GGCCCTCTATTGTCATTGTC-3′ |
| AnSt314 | 5′-CAGGAGTTGCTGAATCGAG-3′ |
| AnSt615 | 5′-GGTATTCATGCCAGAGAATG-3′ |
| AnSt808 | 5′-CAACTTCCCCTTCCAGTG-3′ |
| R1 | 5′-GTCAGCTCGAGCGTGTAC-3′ |
| R2 | 5′-GGTTGATTTATTGCCACTG-3′ |
| AnSt223 | 5′-CCGTAACTTCCCTTTCATG-3′ |
| AnSt297 | 5′-CCGAGAACGAAACCAAAG-3′ |
| AnSt408 | 5′-ACGACTTCCTGCACGCAC-3′ |
| AnSt440 | 5′-CTGCAGCGTTTGGGTGAG-3′ |
| R427 | 5′-TGCAGATCTTCCGCGTTC-3′ |
| R328 | 5′-CGCCTTGTACTGGTCCATC-3′ |
| R74 | 5′-AGCTGTTCCGCATAGTCTTC-3′ |
| As.RT.F | 5′-GAGGATGTACAGGAGTTGCTGAAC-3′ |
| As.RT.R | 5′-CGTCCAGAAGTGTTCAAAGTTCAC-3′ |
| S7F | 5′-TGGAAATGAACTCGGATCTGAAG-3′ |
| S7R | 5′-ACCGGCACGTAGATGATGATC-3′ |
| ClCPBAs-F | 5′-GCTCTCGGATCCGGTTCCTATCATGAATTTGAAGTG-3′ |
| ClCPBAs-R | 5′-CTCAGGCTCGAGCGGCGTCAACGACAACGT-3′ |
a
Bold letters and underlining are related to the BamHI and XhoI recognition sites in cloning primers.