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Linear DNA dose dependence of transformation of S. infantarius strain 3AG-1 by a genomic marker. Strain 3AG-1ΔcomS growing in CDM at an OD of 0.5 was exposed to SinComS9–15 (1 μM) and the indicated amounts of donor DNA in 250-μl volumes. After 40 min at 37°C, samples were diluted in M17 medium for expression and plating. The dashed line indicates linear regression fit to data between 0 and 1 μg of DNA/ml. Error bars represent standard deviations among triplicate determinations.
