Table 2.
Oligonucleotide primers used in this study
| Primer | DNA sequencea |
|---|---|
| W73 | CAATAAAATGGTTGGTTGAAGGTCGTG |
| W152 | CCGAGTCGACCAAGTACAAAGAATTTTAATTTCATCG |
| W153 | CCGATCTAGAACGTTCTTAATATTCTTGATGGGAC |
| W154 | CCGATCTAGAAAACCAGAACCTCGCTTAGGCC |
| W160 | TTTGCGCTCCTTCAACTGGGCA |
| W180 | CCGAGTCGACCCTCCTGTATTTGACGTAACGTG |
| W181 | CCGAGTCGACTAATGTGGTTGTCCGATTAAGGAC |
| W201 | TTACTTCTAGACCTAAGTGGAATGTCTCCACGG |
| W202 | TTACTTCTAGACTCAAATATAAACATTACCATGAAC |
| W203 | TTACTTCTAGAGGACTAATCATTTTAATCTCTATACTC |
| W291 | CCGAGTCGACGGATAAGCTATTGTCCTTATTCCAC |
| W336 | ATTTTGTCGACGAAGTATTCAATCAAACAATTACACC |
| W355 | GTGGGTAGGATCAATAAAGACGTTTTATATGTGTCTGC |
| W356 | GTAATATGGACACAGGCCTAAGCG |
| W357 | CCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCAATAAAGACGTTTTATATGTGTCTGC |
| M13 | GAGCGGATAACAATTTCACACAGG |
a
Underlined sequences represent unique restriction sites introduced for cloning purposes.