Table 3.
Analysis of DNA sequences found at the ospZ and icsP locus in Shigella spp.a
| Species, serotype, and strain name | GenBank accession no., NCBI reference sequence no., or source of strain | OspZ product (full-length, 230 aa) and coordinates of gene on published sequence | Identity to ospZ and icsP intergenic region taken from 2457T (%) | Description of sequence change in intergenic region |
|---|---|---|---|---|
| S. boydii, Sb227 | CP000037 | Full length (8); complement (123143–123835) | 97.0 | 45-bp deletion between −270 and −225 relative to ospZ gene, and 3 single point substitutions |
| S. boydii CDC3083-94 | NC_010660.1 | Full length; complement (206053–206745) | 99.7 | 2 single point substitutions |
| S. dysenteriae serotype 1, Sd197 | CP000035.1 | Full length (7); complement (179127–179819) | 99.7 | 4 single point substitutions |
| S. dysenteriae serotype 2, (10)4279 | This work | Full length | 97 | 45-bp deletion between −270 and −225 relative to ospZ gene, and 3 single point substitutions |
| S. flexneri serotype 2a, 2104 | RRB strain | 42-aa deletion, truncated to amino acid 188 (8) | 100 | NA |
| S. flexneri serotype 6, 0106164 | RRB strain | Full length (8) | 99.7 | Single point substitution |
| S. flexneri serotype 2a, 2457T | This work | 42-aa deletion, truncated to amino acid 188 | Reference sequence | NA |
| S. flexneri serotype 2a, pCP301 | AF386526.1 | 42-aa deletion, truncated to amino acid 188; complement (218144–218711) | 100 | NA |
| S. flexneri serotype 5a, M90T pWR501 | AF348706.1 | 42-aa deletion, truncated to amino acid 188; complement (218378–218941) | 99.9 | 2 single point substitutions |
| S. flexneri serotype 5a, M90T pWR100 | AL391753.1 | 42-aa deletion, truncated to amino acid 188; complement (212330–212896) | 99.9 | 2 single point substitutions |
| S. sonnei, Ss046 | NC_007385.1 | Full length (8); complement (210796–211488) | 99.7 | 2 single point substitutions and 1 insertion |
a
aa, amino acid(s); NA, not applicable.