Fig 1.

Detection of tatD, rfaH, and ubiD gene expression in E058 and E058ΔrfaH by RT-PCR. The RT-PCRs were performed using the following templates: cDNA derived from total RNA of E058 (lanes 1) and E058ΔrfaH (lanes 4) and genomic DNA from E058 (lanes 2) and E058ΔrfaH (lanes 5). Reaction sets contained the following primers: for lanes labeled “a,” TF/TR; for lanes labeled “b,” HF/HR; and for lanes labeled “c,” UF/UR. The negative controls (lanes 3 and 6) used total RNA as the template without activation of RT. A 200-bp marker (TaKaRa) was used as the molecular size standard (M).