Table 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Characteristics | Source or reference |
|---|---|---|
| Strains | ||
| E058 | Wild-type avian E. coli serotype O2 | 41 |
| E058ΔrfaH | E058ΔrfaH::cat | This study |
| ReE058ΔrfaH | complementation of E058ΔrfaH | This study |
| DH5α | λ− ϕ80dlacZΔM15 Δ(lacZYA-argF)U169 recA1 endA1 hsdR17(rK− mK−) supE44 thi-1 gyrA relA1 | Invitrogen |
| Plasmids | ||
| pMD18-T Vector | TA cloning vector; Amp | TaKaRa |
| pGEM-T Easy Vector | TA cloning vector; Amp | Promega |
| pMD-rfaH | rfaH cloned into pMD 18-T vector | This study |
| pMD-rfaH-cat | Cat-resistant gene inserted into pMD-rfaH | This study |
| pT-PnativerfaH | pGEM-T Easy carrying rfaH ORF and its putative native promoter | This study |
| pKD46 | Amp; expresses λ Red recombinase | 23 |
| pKD3 | cat gene, template plasmid | 23 |