Table 2.
Primers designed and used in this study
| Primer | Primer sequence (5′–3′) | Target gene, plasmid, or region |
|---|---|---|
| Targets of primers for gene mutagenesis | ||
| HF | CTCGGATCCTTAGAGTTTGCGGAACTC (BamHI site underlined) | rfaH |
| HR | CTCAAGCTTTTACTGTACTGCAAGCGC (HindIII site underlined) | rfaH |
| SHF | CTCGATATCGCCGTATCCTGGTGATAA (EcoRV site underlined) | pMD-rfaH |
| SHR | CTCGATATCGTGGTATGAATCACTTCC (EcoRV site underlined) | pMD-rfaH |
| CF | CTCGATATCTTGTGTAGGCTGGAGCTGCT (EcoRV site underlined) | pKD3 |
| CR | CTCGATATCATGGGAATTAGCCATGGTCC (EcoRV site underlined) | pKD3 |
| RHF | CTGCGGCAGTTTATCAAACAG | Upstream region of rfaH |
| RHR | GAAGCGATTATTGAGCTGGCC | Downstream region of rfaH |
| TF | ATGGGCTACTCATCACCG | tatD |
| TR | CACCAGGCAGTTTATCCAG | tatD |
| UF | CGAAAACCCTAAAGGCTACT | ubiD |
| UR | CCACTCCTGATAATCCAACG | ubiD |
| Targets of primers for real-time PCR | ||
| rfaH RT-F | TCGGGCTCGGTGAAA ATG | rfaH |
| rfaH RT-R | ATCGACGCCGTATCCTGGTG | rfaH |
| chuA RT-F | TACCGACCCAACCAACAG | chuA |
| chuA RT-R | GTCCCGAACGCCAAAAT | chuA |
| kpsM RT-F | TGCCAGACATCTCGTTCC | kpsM |
| kpsM RT-R | TCGCTTCAATAGCACCAAT | kpsM |
| traT RT-F | CAGCAATCAAGAAGCGTAAC | traT |
| traT RT-R | TTCGCCTGAATCCAGTAGTA | traT |
| iutA RT-F | CGATGCCACCTTGCTTGA | iutA |
| iutA RT-R | CAGCCCGTTACTGACGAATG | iutA |
| iucD RT-F | AACAACCTTATTTACCACCCTG | iucD |
| iucD RT-R | TCTGTCCTCCACCAACCAC | iucD |
| iroN RT-F | GGCGATACGCAAAACAGT | iroN |
| iroN RT-R | CCCAGTCCCAGATACCATT | iroN |
| iss RT-F | AACACCAAAGGAAACCATCA | iss |
| iss RT-R | CCGAGCAATCCATTTACGA | iss |
| tsh RT-F | GGCGCTTACTTTGATGTGAT | tsh |
| tsh RT-R | GCTGTTACGACGCATTGAGA | tsh |
| vat RT-F | GCTTTGGTTCGCTCGTGTT | vat |
| vat RT-R | ATCTGCCTGGATGGTTGTGTT | vat |
| cvaC RT-F | AATGTCTCCATCCGGTTTA | cvaC |
| cvaC RT-R | TCTTCCCGCAGCATAGTT | cvaC |
| astA RT-F | AAA AGTCGGCTGGTGGAA | astA |
| astA RT-R | CTGCGTGGCATTTGAGGA | astA |