Table 2.
Strains and plasmids used in this study
| Strain or plasmid | Description | Source or reference |
|---|---|---|
| Strains | ||
| EPEC E2348/69 | Wild-type E. coli O127:H6 isolate | 39 |
| E2348/69 ΔescE | EPEC escE deletion mutant | This study |
| E2348/69ΔescG | EPEC escG deletion mutant | This study |
| Plasmids | ||
| pEscE-2HA | C-terminally tagged EscE in pTOPO-2HA | This study |
| pEscG-2HA | C-terminally tagged EscG in pTOPO-2HA | This study |
| pEscG(C68S)-2HA | C-terminally tagged EscG mutated at position 68 in pTOPO-2HA | This study |
| pHis-EscF | N-terminally His tagged EscF in pET28a | This study |
| pEscF | EscF in pET21a | This study |
| pEscFΔ5aa | Truncated EscF in pET21 (deletion of 5 aa at the C terminus) | This study |
| pHis-EscE | N-terminally His tagged EscE in pET28a | This study |
| pHis-EscG | N-terminally His tagged EscG in pET28a | This study |
| pHis-EscE-EscG | N-terminally His tagged EscE and untagged EscG in pET28a | This study |
| pHis-EscG-EscE | N-terminally His tagged EscG and untagged EscE in pET28a | This study |
| pCR2.1-TOPO | PCR cloning vector; Ampr Kanr | Invitrogen |
| pET28a(+) | Expression vector for His tagging; Kanr | Novagen |
| pET21a(+) | Expression vector; Ampr | Novagen |
| pTOPO-2HA | Expression vector for HA tagging; Kanr | 25 |
| pACYC184 | Cloning vector; Cmr Tcr | 40 |
| pRE112 | Suicide vector for allelic exchange; Cmr | 24 |