Fig 7.

Effect of FPSS on in vitro transcription of the L. monocytogenes σ^B-dependent promoter. (Left lanes) B. subtilis RNAP (150 nM) was added to reaction mixtures containing various concentrations of σ^B (37.5, 75, 150, 225, or 300 nM) in the presence of DMSO (lanes 1 to 5) or 100 μM FPSS (lanes 6 to 10). Control reactions (lanes 12 and 13) exhibit sigma factor-initiated transcription from both promoters, even in the presence of DMSO (lanes 14 and 15). (Right lanes) B. subtilis RNAP (150 nM) was added to reaction mixtures containing σ^B (150 nM) in the presence of DMSO (lane 16) or various concentrations of FPSS (25, 50, 75, 100, 125, or 150 μM) (lanes 17 to 22).