Table 1.
Plasmids and strains used in this study
| Plasmid or strain | Relevant genotype | Source |
|---|---|---|
| Plasmids | ||
| pDLR1 | Pfri Lm SigA2 SigB in pUC19; Apr | This study |
| pDLR2 | Pspac rsbVBs; Neor | This study |
| pCK35 | Pspac Δ(rsbR rsbS) rsbT+; Neor | 17 |
| pUC19 | High-copy-number cloning vector; Apr | 28 |
| pQE-30 | N-terminal His6 expression vector; PT5/Olac; ColEI ori; Apr | Qiagen |
| Strains | ||
| B. subtilis | ||
| FSL B2-303 | Pspac rsbVBs amyE::ctc-lacZ trpC2 | pDLR2→PB198 |
| FSL B2-304 | Pspac rsbVBs amyE::ctc-lacZ trpC2 sigBΔ3::spc trpC2 | pDLR2→PB345 |
| PB2 | trpC2 | 29 |
| PB198 | amyE::ctc-lacZ trpC2 | 30 |
| PB206 | rsbVΔ1 amyE::pDH32-ctc | 30 |
| PB213 | Pspac (rsbV+ rsbWΔ1 sigB+ rsbX+) amyE::pDH32-ctc trpC2 | 30 |
| PB345 | amyE::ctc-lacZ trpC2 sigBΔ3::spc trpC2 | 31 |
| E. coli | ||
| FSL B2-273 | M15 pREP4 pQE-30 His6-SigALm | 32 |
| FSL B2-274 | M15 pREP4 pQE-30 His6-SigBLm | 32 |
| FSL B2-302 | TOP10 pUC19-Pfri Lm | pDLR1→TOP10 |
| TOP10 | F− mcrA Δ(mrr-hsdRMS-mcrBC) ϕ80lacZΔM15 ΔlacX74 recA1 araD139 Δ(ara-leu)7697 galU galK rpsL (Strr) endA1 nupG | Invitrogen |
| L. monocytogenes | ||
| 10403S | Wild type; serotype 1/2a | 33 |
| 10403SΔsigB | ΔsigB | 34 |