Fig 3.

Western analysis of AniA accumulation during oxygen-limited growth in the presence of nitrite of the Δc₂ Δc₄ and Δc₂ Δc₅ double mutants compared with the parent. N. gonorrhoeae JCGC853 (Δc₂ Δc₄) (A) and JCGC852 (Δc₂ Δc₅) (B) strains were grown under oxygen-limited conditions compared with the parent strain in the presence of 1.1 mM or 2 mM nitrite, respectively. Samples were taken during growth, separated by SDS-PAGE, and blotted onto PVDF membranes, and AniA protein was detected using anti-AniA antibodies and ECL-Plus chemiluminescent labeling.