Table 3.
Rates of nitrite reduction by cytochrome mutants of N. gonorrhoeaea
| Strain | Genotype | Rate of nitrite reduction | % of parental rate | P value(s) |
|---|---|---|---|---|
| F62 | Parental | 222 ± 8 | 100 | |
| AHGC101 | ccoP-C368A | 115 ± 8 | 52 | 1.58 × 10−9 (F62) |
| JCGC850 | Δc5 | 130 ± 7 | 59 | 1.37 × 10−9 (F62) |
| JCGC310 | c5Δ200–279 | 87 ± 9 | 39 | 1.26 × 10−8 (F62), 0.003 (Δc5) |
| JCGC320 | c5Δ200–279 ccoP-C368A | 26 ± 1.3 | 12 | 0.001 (c5Δ200–279) |
| JCGC851 | Δc2 | 115 ± 7 | 52 | 1.37 × 10−12 (F62) |
| JCGC852 | Δc2 Δc5 | 86 ± 9 | 39 | 9.14 × 10−4 (Δc5) |
| JCGC330 | Δc2 ccoP-C368A | 115 ± 15 | 52 | 0.97 (ccoP-C368A) |
| JCGC800 | Δc4 | 81 ± 6 | 36 | 1.66 × 10−13 (F62) |
| JCGC853 | Δc4 Δc2 | 93 ± 5 | 42 | 0.17 (Δc4) |
| JCGC340 | Δc4 ccoP-C368A | 79 ± 7.7 | 36 | 0.81 (Δc4), 0.004 (ccoP-C368A) |
| JCGC142 | ΔaspC-lctP::plac-cccA+ Δc2 | 119 ± 3 | 54 | 0.71 (Δc2) |
| JCGC142 | ΔaspC-lctP::plac-cccA+ Δc2 + IPTG | 128 ± 5 | 58 | 0.56 (ΔaspC-lctP::plac-cccA+ Δc2) |
a
Units for rates of nitrite reduction are nmol NO2− reduced min−1 mg dry cell mass−1; values are means ± standard error of the means. P values are relative to the strain shown in parentheses. The data are based upon results of between 5 and 29 independent experiments. Note that Δc5 indicates that the complete coding sequence for cytochrome c5 has been deleted, but c5Δ200–279 lacks only the last 80 codons, which include the second heme-binding domain of cytochrome c5.