Fig 2.

Effect of unlabeled competitors on [¹⁴C]maltose uptake rates in C. glutamicum (A) and growth (filled symbols) and substrate consumption (open symbols) of C. glutamicum WT (circles) and C. glutamicum Δmus in CgC minimal medium with 1% (wt/vol) maltotriose as the sole carbon source (B). One representative growth curve of at least three independent cultivations is shown; the results of each of the cultivations were comparable. The uptake measurements were started by simultaneous addition of 50 μM [¹⁴C]maltose and 5 mM competitor. As a control, [¹⁴C]maltose uptake was measured without competitor. Cells for the experiments were cultivated in CgC minimal medium with 1% (wt/vol) glucose. The data represent mean values and standard deviations of three independent measurements from two independent cultivations.