Fig 6.

Analysis of the replication capacities of HSV-1 mutants lacking miR-H2, miR-H3, or miR-H4. Each recombinant virus and wild-type HSV-1 (strain 17syn+) were used to infect two different types of cultured cells, Neuro2A cells (A and B) or NIH 3T3 cells (C and D), at a low MOI of 0.01 (A and C) or a high MOI of 3 (B and D). In each case, results are shown for the 17mH2 (green) (top), 17mH3 (purple) (center), and 17dH4 (light blue) (bottom) recombinant virus clones compared with WT 17syn+ (blue). Infected cells were harvested at the indicated hours postinfection. Levels of viral DNA were determined by quantitative real-time PCR using primers and a probe within the viral polymerase gene (UL30) and were plotted as ratios to the corresponding values for uninfected cells (0 h postinfection). The standard errors of the means were from three independent experiments.