Table 2.

qRT-PCR analysis of the levels of expression of HSV-1 microRNAs in infected 293 cells^a

Time postinfection (h)	No. of microRNA strands per cell
	miR-H1-5p	miR-H2-3p	miR-H3-3p	miR-H6-3p
2	60	14	62	143
4	302	5	70	272
6	3,562	15	88	742
8	5,254	34	95	1,629
10	7,172	36	95	2,772
12	9,140	49	127	3,923
24	9,384	46	139	3,941

^a293 cells were infected with HSV-1 strain 17syn+, and samples were harvested at the indicated times postinfection. A total of 10⁶ cells were used for RNA isolation, and the number of miRNA strands per cell was quantitated by qRT-PCR by comparison to standard curves obtained using synthetic versions of these HSV-1 miRNAs.