Table 5.

Relative recovery levels of selected HSV-1 microRNAs

HSV-1 miRNA	Relative level of strandsa in:			Ratio of relative recovery in total RNA to that in RISC-associated RNAb determined by:
	Total RNA (determined by qRT-PCR)	RISC-associated RNA		qRT-PCR	Deep sequencing
		Determined by qRT-PCR	Determined by deep sequencing
miR-H1-5p	87	0.44	0.11	198	791
mir-H2-3p	2.2	0.52	0.45	4.2	4.9
miR-H3-3p	1.0	1.0	1.0	1.0	1.0
miR-H6-3p	10.4	1.1	1.2	9.5	8.7

^aRecovered from Neuro2A cells infected (MOI, 2) with the HSV-1 isolate 17syn+. RNA was isolated at 8 h postinfection. The number of strands of each HSV-1 miRNA in a total small-RNA preparation or in an RNA sample isolated from a RISC immunoprecipitate from the same culture was normalized to the quantity of miR-H3-3p strands, which was set at 1.

^bThe miR-H3-3p ratio was set at 1.0. The higher the value, the lower the level of recruitment of the indicated HSV-1 miRNA into the RISC.