Fig 9.

Intravenous VSV-rp30 targets multiple subcutaneous melanoma xenografts. SCID mice bearing multiple subcutaneous rYUMAC melanoma xenografts were given a single tail vein injection of VSV-rp30 (1 × 10⁸ PFU/100 μl). (A) Skin of the back and flank section with bilateral xenografts, with the subcutaneous (s.c.) side facing up. Human YUMAC melanoma cells modified to express red fluorescent protein (RFP) allow tracing of tumor masses surrounded by normal tissue, as illustrated by stereomicroscope images (B and C). Green filter images confirm VSV-rp30 infection restricted to red tumor masses at 5 dpi. Microsections of individual tumors reveal details of close adherence of viral intratumoral spread to the tumor border, as seen in green-red merged low-magnification images (D and G) and high-magnification details (E, F, H, I).