Fig 2.

Vpu, Vpr, and Vif do not affect the level of IRF-3 expression. (A) 293T cells were transfected with 5 μg of pCG vectors coexpressing NL4-3 Vpu, Vpr, or Vif proteins and eGFP via an IRES. A vector coexpressing NCDV NSP1 and yellow fluorescent protein (YFP) was used as a positive control. The cells were permeabilized at 24 h posttransfection and stained for IRF-3 (Santa Cruz Biotechnology) for FACS analysis. (B) Levels of IRF-3 expression in the presence of the indicated expression constructs relative to the empty vector control (set as 100%). Data are mean values (± the standard errors of the means) derived from three independent experiments.