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Identification of the hnRNP binding site in the NiV N 3′ UTR. The hnRNP binding site was identified using RNA pulldown assays in the presence of competitors of different lengths. RNAs corresponding to the full-length NiV N 3′ UTR (586 nt) were labeled at the 3′ end with biotin and used as RNA probes. hnRNP protein was detected by Western blotting.
