Fig 6.

Expression of NiV N 3′ UTR reporters was increased by knockdown of hnRNP D. (A) siRNAs designed to knock down hnRNP A/B, hnRNP D, or EGFP (designated sihnRNP A/B, sihnRNP D, or siEGFP) were transfected into HeLa cells. The membranes were cut at the 50-kDa marker before addition of antibody. (B) Expression levels of NiV N 3′ UTR reporters when hnRNP A/B or D was repressed. Luciferase activities were measured and normalized to Renilla luciferase activities. The value of siEGFP-transfected cells was set to 100. The error bars indicate standard deviations. (C) Expression levels of six NiV UTR reporters when hnRNP D was repressed. The six NiV 3′ UTR reporters were transfected into hnRNP D KD cells. Luciferase activities were measured and normalized to Renilla luciferase activities. The value of siEGFP-transfected cells was set to 100.