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Effect of exogenous La on HCV replication and translation. (A) Huh7 cells were cotransfected with wild-type or mutated pSGR-JFH1/Luc RNA and a construct expressing La/P4La (mutant La). The cells were harvested at 24 h posttransfection, and replication was checked by semiquantitative RT-PCR (“C” denotes control, “Wt” denotes wild type, and “Mut” denotes mutant). (B) A luciferase assay was also performed to check HCV IRES-mediated translation.
