Fig 4.

A fraction of CD11c⁺ cells producing IFN-β in lungs of mice infected with wild-type SC35M are macrophages. Lungs of virus-infected “global” reporter mice were harvested at 48 h postinfection and stained simultaneously with antibodies against luciferase (luc) and CD11c and with lectin BS-1. Luciferase-positive cells were positive for both CD11c and the lung macrophage marker BS-1 (A) or for CD11c but not BS-1 (B). Counterstaining (blue) was done with DAPI. Size bars, 10 μm.