Table 1.
EEHV1 ORF | HCMV ortholog | Identitya (%) | ORF family | Protein nameb | Protein description |
---|---|---|---|---|---|
EE1 | 95 | Protein EE1 | |||
EE2 | 99 | Protein EE2 | |||
EE3 | 32 | EE3 | Protein EE3 | Contains signal peptide | |
EE4 | 42 | EE3 | Protein EE4 | Contains signal peptide | |
EE5 | 51 | Protein EE5 | Contains signal peptide | ||
U82 | UL115 | 70 | Envelope glycoprotein L | Contains signal peptide; complexed with envelope glycoprotein H; involved in cell entry; involved in cell-to-cell spread | |
U81 | UL114 | 73 | Uracil-DNA glycosylase | Involved in DNA repair | |
EE6c | Protein EE6 | Contains signal peptide | |||
U79 | UL112 | 89 | Protein UL112 | Involved in gene regulation; involved in DNA replication | |
U77 | UL105 | 100 | Helicase-primase helicase subunit | Involved in DNA replication | |
U76 | UL104 | 100 | Capsid portal protein | Dodecamer located at one capsid vertex in place of penton; involved in DNA encapsidation | |
U75 | UL103 | 98 | Tegument protein UL7* | Involved in virion morphogenesis | |
U74 | UL102 | 98 | Helicase-primase subunit | Involved in DNA replication | |
U73 | 98 | DNA replication origin-binding helicase | Involved in DNA replication | ||
U72 | UL100 | 97 | Envelope glycoprotein M | Type 3 membrane protein; 8 transmembrane domains; complexed with envelope glycoprotein N; involved in virion morphogenesis; involved in membrane fusion | |
U71 | UL99 | 91 | Myristylated tegument protein | Envelope-associated; involved in virion morphogenesis | |
U70 | UL98 | 98 | DNase | Involved in DNA processing | |
U69 | UL97 | 100 | Tegument serine/threonine protein kinase | Involved in protein phosphorylation | |
U68 | UL96 | 100 | Tegument protein UL14* | Involved in virion morphogenesis | |
U67 | UL95 | 100 | Protein UL95 | Promotes accumulation of late transcripts | |
U65 | UL94 | 96 | Tegument protein UL16* | Possibly involved in virion morphogenesis | |
U64 | UL93 | 99 | DNA packaging tegument protein UL17* | Capsid-associated; involved in DNA encapsidation; involved in capsid transport | |
U63 | UL92 | 99 | Protein UL92 | ||
U62 | UL91 | 99 | Protein UL91 | ||
U60 | UL89 | 99 | DNA packaging terminase subunit 1 | Contains ATPase domain; involved in DNA encapsidation | |
U59 | UL88 | 100 | Tegument protein UL88 | ||
U58 | UL87 | 100 | Protein UL87 | Promotes accumulation of late transcripts | |
U57 | UL86 | 99 | Major capsid protein | 6 copies form hexons, 5 copies form pentons; involved in capsid morphogenesis | |
U56 | UL85 | 99 | Capsid triplex subunit 2 | Complexed 2:1 with capsid triplex subunit 1 to connect capsid hexons and pentons; involved in capsid morphogenesis | |
U54 | UL82/UL83/UL84 | 98 | DURP | Protein U54 | |
U53.5 | UL80.5 | 100 | Capsid scaffold protein | Clipped near C terminus; involved in capsid morphogenesis | |
U53 | UL80 | 100 | Capsid maturation protease | Serine protease (N-terminal region); minor scaffold protein (remainder of protein, clipped near C terminus); involved in capsid morphogenesis | |
U52 | UL79 | 100 | Protein UL79 | Promotes accumulation of late transcripts | |
U51 | UL78 | 98 | Envelope protein UL78 | Type 3 membrane protein; 7 transmembrane domains; similar to GPCRs; putative chemokine receptor; possibly involved in intracellular signaling | |
U50 | UL77 | 98 | DNA packaging tegument protein UL25* | Located on capsid near vertices; possibly stabilizes the capsid and retains the genome; involved in DNA encapsidation | |
U49 | UL76 | 100 | Nuclear protein UL24* | ||
EE7 | 82 | Thymidine kinase | Involved in nucleotide metabolism | ||
U48 | UL75 | 66 | Envelope glycoprotein H | Type 1 membrane protein; possible membrane fusogen; complexed with envelope glycoprotein L; involved in cell entry; involved in cell-to-cell spread | |
U47 | UL74 | 63 | Envelope glycoprotein O | Associated with envelope glycoprotein H and envelope glycoprotein L; involved in virion morphogenesis | |
U46 | UL73 | 82 | Envelope glycoprotein N | Type 1 membrane protein; complexed with envelope glycoprotein M; involved in virion morphogenesis; involved in membrane fusion | |
EE8 | 74 | Protein EE8 | Contains potential transmembrane domain | ||
U27 | UL44 | 99 | DNA polymerase processivity subunit | dsDNA-binding protein; involved in DNA replication | |
EE9 | 100 | Ribonucleotide reductase subunit 2 | Involved in nucleotide metabolism | ||
U28 | UL45 | 100 | Ribonucleotide reductase subunit 1 | Involved in nucleotide metabolism | |
U29 | UL46 | 99 | Capsid triplex subunit 1 | Complexed 1:2 with capsid triplex subunit 2 to connect capsid hexons and pentons; involved in capsid morphogenesis | |
U30 | UL47 | 98 | Tegument protein UL37* | Complexed with large tegument protein; involved in virion morphogenesis | |
U31 | UL48 | 96 | Large tegument protein | Complexed with tegument protein UL37; ubiquitin-specific protease (N-terminal region); involved in capsid transport | |
U32 | UL48A | 89 | Small capsid protein | Located externally on capsid hexons; involved in capsid morphogenesis; possibly involved in capsid transport | |
U33 | UL49 | 99 | Protein UL49 | ||
U34 | UL50 | 100 | Nuclear egress membrane protein | Type 2 membrane protein; interacts with nuclear egress lamina protein; involved in nuclear egress | |
U35 | UL51 | 100 | DNA packaging protein UL33* | Interacts with DNA packaging terminase subunit 2; involved in DNA encapsidation | |
U36 | UL52 | 100 | DNA packaging protein UL32* | Involved in DNA encapsidation; possibly involved in capsid transport | |
U37 | UL53 | 100 | Nuclear egress lamina protein | Interacts with nuclear egress membrane protein; involved in nuclear egress | |
U38 | UL54 | 97 | DNA polymerase catalytic subunit | Involved in DNA replication | |
U39 | UL55 | 87 | Envelope glycoprotein B | Type 1 membrane protein; possible membrane fusogen; binds cell surface heparan sulfate; involved in cell entry; involved in cell-to-cell spread | |
U40 | UL56 | 100 | DNA packaging terminase subunit 2 | Involved in DNA encapsidation | |
U41 | UL57 | 100 | Single-stranded DNA-binding protein | Contains zinc finger; involved in DNA replication; possibly involved in gene regulation | |
U42 | UL69 | 100 | Multifunctional expression regulator | RNA-binding protein; shuttles between nucleus and cytoplasm; inhibits pre-mRNA splicing; exports virus mRNA from nucleus; exerts most effects posttranscriptionally; involved in gene regulation; involved in RNA metabolism and transport | |
U43 | UL70 | 99 | Helicase-primase primase subunit | Involved in DNA replication | |
U44 | UL71 | 100 | Tegument protein UL51* | Involved in virion morphogenesis | |
EE10 | 99 | Protein EE10 | |||
EE11 | 100 | UL27 | Protein EE11 | ||
U4 | UL27 | 100 | UL27 | Protein UL27 | |
U11 | UL32 | 99 | Tegument protein pp150 | Major tegument protein; binds to capsids | |
U12 | UL33 | 100 | Envelope glycoprotein UL33 | Type 3 membrane protein; 7 transmembrane domains; similar to GPCRs; putative chemokine receptor; involved in intracellular signaling | |
UL34 | UL34 | 100 | Protein UL34 | Involved in gene regulation | |
U14 | UL35 | 95 | Tegument protein UL35 | ||
EE12 | 94 | Protein EE12 | Contains potential transmembrane domain | ||
EE13 | 100 | Protein EE13 | Contains US22 domain | ||
EE14 | 100 | Protein EE14 | |||
EE15 | 90 | Protein EE15 | Contains potential transmembrane domain | ||
EE16 | 62 | Protein EE16 | Contains signal peptide | ||
EE17 | 72 | Protein EE17 | Contains signal peptide | ||
EE18 | 99 | 7TM | Membrane protein EE18 | Type 3 membrane protein; 7 transmembrane domains | |
EE19 | 99 | 7TM | Membrane protein EE19 | Type 3 membrane protein; 7 transmembrane domains | |
EE20 | 100 | EE20 | Membrane protein EE20 | Type 2 membrane protein | |
EE21 | 99 | 7TM | Membrane protein EE21 | Type 3 membrane protein; 7 transmembrane domains; similar to GPCRs | |
EE22 | 78 | OX-2 | Membrane protein EE22 | Type 2 membrane protein; contains 2 immunoglobulin domains; similar to OX-2 (CD200) | |
EE23 | 100 | OX-2 | Protein EE23 | Contains signal peptide; contains immunoglobulin domain; similar to OX-2 (CD200) | |
EE24 | 100 | Protein EE24 | Contains potential transmembrane domain | ||
EE25 | 99 | Protein EE25 | Contains potential transmembrane domain | ||
EE26 | 94 | 7TM | Membrane protein EE26 | Type 3 membrane protein; 7 transmembrane domains; similar to GPCRs | |
EE27 | 100 | Protein EE27 | |||
EE28 | 100 | 7TM | Membrane protein EE28 | Type 3 membrane protein; 7 transmembrane domains; similar to GPCRs | |
EE29 | 100 | DURP | Protein EE29 | ||
EE30 | 98 | Protein EE30 | |||
EE31 | 96 | 7TM | Membrane protein EE31 | Type 3 membrane protein; 7 transmembrane domains | |
EE32 | 90 | EE20 | Protein EE32 | Contains potential transmembrane domain | |
EE33 | 100 | 7TM | Membrane protein EE33 | Type 3 membrane protein; 7 transmembrane domains | |
EE34 | 99 | 7TM | Membrane protein EE34 | Type 3 membrane protein; 7 transmembrane domains; similar to GPCRs | |
EE35 | 100 | 7TM | Membrane protein EE35 | Type 3 membrane protein; 6 transmembrane domains | |
EE36 | 99 | 7TM | Membrane protein EE36 | Type 3 membrane protein; 7 transmembrane domains | |
EE37 | 100 | 7TM | Membrane protein EE37 | Type 3 membrane protein; 7 transmembrane domains | |
EE38 | 100 | 7TM | Membrane protein EE38 | Type 3 membrane protein; 7 transmembrane domains | |
EE39 | 99 | 7TM | Membrane protein EE39 | Type 3 membrane protein; 7 transmembrane domains | |
EE40e | 97g | 7TM | Membrane protein EE40 | Type 3 membrane protein; 7 transmembrane domains | |
EE41 | 99 | 7TM | Membrane protein EE41 | Type 3 membrane protein; 7 transmembrane domains | |
EE42 | 100 | 7TM | Membrane protein EE42 | Type 3 membrane protein; 7 transmembrane domains | |
EE43 | 100 | 7TM | Membrane protein EE43 | Type 3 membrane protein; 7 transmembrane domains | |
EE44e,f | 75g | Protein EE44 | Contains signal peptide | ||
EE45 | 93 | 7TM | Membrane protein EE45 | Type 3 membrane protein; 8 transmembrane domains; similar to GPCRs | |
EE46 | 99 | β-1,3-Galactosyl-O-glycosyl-glycoprotein β-1,6-N-acetylglucosaminyltransferase | Contains potential transmembrane domain; involved in protein glycosylation | ||
EE47 | 95 | 7TM | Membrane protein EE47 | Type 3 membrane protein; 8 transmembrane domains; similar to GPCRs | |
EE48 | 99 | 7TM | Membrane protein EE48 | Type 3 membrane protein; 7 transmembrane domains | |
EE49 | 96 | 7TM | Membrane protein EE49 | Type 3 membrane protein; 8 transmembrane domains | |
EE50 | 100 | EE50 | Membrane protein EE50 | Contains signal peptide; contains immunoglobulin domain | |
EE51 | 97 | OX-2 | Membrane protein EE51 | Type 2 membrane protein; contains 2 immunoglobulin domains; similar to OX-2 (CD200) | |
EE52 | 88 | EE50 | Membrane protein EE52 | Type 2 membrane protein; contains immunoglobulin domain | |
EE53f | 45g | EE50 | Membrane protein EE53 | Type 2 membrane protein; contains immunoglobulin domain | |
EE54d | EE50 | Membrane protein EE54 | Type 2 membrane protein | ||
EE55d,e | EE50 | Membrane protein EE55 | Type 2 membrane protein | ||
EE56d | EE50 | Membrane protein EE56 | Type 2 membrane protein | ||
EE57e,f | 82g | EE50 | Membrane protein EE57 | Type 2 membrane protein; contains immunoglobulin domain | |
EE58e | 51g | EE50 | Membrane protein EE58 | Type 2 membrane protein | |
EE59f,h | 7TM | Membrane protein EE59 | Type 3 membrane protein; 7 transmembrane domains | ||
EE60c,f | EE50 | Membrane protein EE60 | Type 2 membrane protein; contains immunoglobulin domain | ||
EE61c,f | EE50 | Membrane protein EE61 | Type 2 membrane protein | ||
EE62c | 7TM | Membrane protein EE62 | Type 3 membrane protein; 7 transmembrane domains | ||
EE63 | 58 | α-(1,3)-Fucosyltransferase | Contains potential transmembrane domain; involved in protein glycosylation |
Calculated from EEHV1A and EEHV1B amino acid sequences aligned by using GCG Gap and shown to the nearest integer.
The standard nomenclature for orthologous herpesvirus proteins employed in NCBI Reference Sequence files is used for conserved ORFs. In this system, the names of proteins specified by certain ORFs are derived from HCMV nomenclature (e.g., tegument protein UL35, encoded by U14). However, the names of proteins specified by certain core ORFs are derived from nomenclature of the alphaherpesvirus herpes simplex virus type 1 (e.g., DNA packaging tegument protein UL25, encoded by U50). Names in the latter category may cause confusion in relation to HCMV ORF nomenclature and are marked by asterisks.
ORF absent from EEHV1B.
ORF absent from EEHV1A.
Fragmented in EEHV1B.
Fragmented in EEHV1A.
Repaired, nonfragmented sequences used.
EEHV1A ORF too fragmented to be repaired.