Fig 1.

EWSR1 precipitates with HCV RNA replication elements. HCV RNAs corresponding to either the CRE, the 3′UTR or the CRE plus+ 3′UTR were incubated with uninfected cellular protein extracts and precipitated via a complementary biotinylated oligonucleotide and streptavidin agarose beads. (A) Silver-stained SDS-PAGE of precipitate. An asterisk (*) denotes the CRE-specific band that was excised for mass spectrometry analysis. (B) Peptide identities, quantities, predicted, and observed molecular mass from the band in panel A that were identified by mass spectrometry. (C) Immunoblot of HCV RNA pull-downs probed for EWSR1. (D) Loading of RNA as bound to beads per pulldown. (E) Quantitation of band densitometry in panel C. This RNA pulldown experiment (C to E) was representative of three independent experiments.